Covalent Organic Frameworks with Chirality Enriched by Biomole cules for Efficient Chiral Separation

GermanEdition:DOI:10.1002/ange.201810571CovalentOrganicFrameworksInternationalEdition:DOI:10.1002/anie.201810571CovalentOrganicFrameworkswithChiralityEnrichedbyBiomole-culesforEfficientChiralSeparationSainanZhang,YunlongZheng,HongdeAn,BrianaAguila,Cheng-XiongYang,YueyueDong,WeiXie,PengCheng,ZhenjieZhang,*YaoChen,*andShengqianMa*Abstract:Theseparationofracemiccompoundsisimportantinmanyfields,suchaspharmacologyandbiology.Takingadvantageoftheintrinsicallystrongchiralenvironmentandspecificinteractionsfeaturedbybiomolecules,herewecon-tributeageneralstrategyisdevelopedtoenrichchiralityintocovalentorganicframeworks(COFs)bycovalentlyimmobi-lizingaseriesofbiomolecules(aminoacids,peptides,enzymes)intoachiralCOFs.Inheritingthestrongchiralityandspecificinteractionsfromtheimmobilizedbiomolecules,theaffordedbiomolecules�COFsserveasversatileandhighlyefficientchiralstationaryphasestowardsvariousracematesinbothnormalandreversephaseofhigh-performanceliquidchromatography(HPLC).Thedifferentinteractionsbetweenenzymesecondarystructureandracemateswererevealedbysurface-enhancedRamanscatteringstudies,accountingfortheobservedchiralseparationcapacityofenzymes�COFs.Chiralresolutionisanimportanttoolintheproductionofpharmaceuticalandbiologicallyactivecompounds,asthebiologicalbehavior,metabolism,andtoxicityofpureenan-tiomersareoftenquitedifferent.[1]Theseparationofracemiccompoundshaslongbeenofgreatsignificance,especiallyforpharmaceuticalandmedicinalapplications,[2,3]whichhasspurredcontinuousinterestinthee