Comparative cytotoxicity of deoxynivalenol, nivalenol, their acetylated derivatives and de-epoxy met

Comparativecytotoxicityofdeoxynivalenol,nivalenol,theiracetylatedderivativesandde-epoxymetabolitesG.SundstølEriksen,H.Pettersson*,T.LundhDepartmentofAnimalNutritionandManagement,SwedishUniversityofAgriculturalSciences,POBox7024,S-75007Uppsala,SwedenReceived16July2003;accepted17November2003AbstractThecytotoxicityofthede-epoxymetabolitesoftrichothecenesnivalenol(NIV)anddeoxynivalenol(DON)wasdeterminedandcomparedwiththecytotoxicityoftherespectivetoxinwithanintactepoxygroupandtheiracetylatedderivatives.Thecytotoxiceffectswasdeterminedbyusingthe5-bromo-20-deoxyuridine(BrdU)incorporationassayassessingDNA-synthesis.ThetoxicityofNIVandDONexpressedastheconcentrationinhibiting50%oftheDNAsynthesis(IC50),wasoccurringatsimilarmicromolarconcentrations(1.19�0.06and1.50�0.34mM).ThetoxicityoffusarenonX(4-acetylNIV)intheassaywassimilartothetoxicityofNIV,andthetoxicityof15-AcDONwasequaltothetoxicityofDON.3-AcDONwaslesstoxicthanDONand15-AcDON.TheIC50valueforde-epoxyDONwas54timeshigherintheassaythantheIC50