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Antioxidant activity of phytoestrogen type isoflavones in biomimetic environments

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606|NewJ.Chem.,2016,40,606--612Thisjournalis©TheRoyalSocietyofChemistryandtheCentreNationaldelaRechercheScientifique2016Citethis:NewJ.Chem.,2016,40,606AntioxidantactivityofphytoestrogentypeisoflavonesinbiomimeticenvironmentsMarianaVoicescu,*aPetraHellwigbandAureliaMegheacTheworkaimstosimulateinvitrotheantioxidantactivityoffourphytoestrogentypeisoflavones,whoseeffectsareclosertothoseofestradiol(daidzein,formononetin,genistein,biochaninA)inbiomimeticenvironments(inlecithinlipidicbi-layersandonsilvernanoparticles),usingthechemiluminescentsystemluminol–hydrogenperoxide,inphosphatebuffer,pH7.4.Thecontributionofacarrierprotein,humanserumalbumin(HSA),totheantioxidantactivityoftheseisoflavoneshasalsobeeninvestigated.TherateofthefluorescencequenchingofHSAbyisoflavoneaswellasthebindingconstantofisoflavonetoHSA,inbothlecithinlipidicbi-layersandonsilvernanoparticles,hasalsobeeninvestigatedbyfluorescencespectroscopy.Theresultsarediscussedwithrelevancetotheoxidativestressprocess.1.IntroductionIsofla

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